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# |
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# BioPerl module for Bio::TreeIO::newick |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Jason Stajich |
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# |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::TreeIO::newick |
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=head1 SYNOPSIS |
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# do not use this module directly |
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use Bio::TreeIO; |
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my $treeio = Bio::TreeIO->new(-format => 'newick', |
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-file => 't/data/LOAD_Ccd1.dnd'); |
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my $tree = $treeio->next_tree; |
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=head1 DESCRIPTION |
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This module handles parsing and writing of Newick/PHYLIP/New Hampshire format. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to the |
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Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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53
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=head2 Reporting Bugs |
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55
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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61
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=head1 AUTHOR - Jason Stajich |
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Email jason-at-bioperl-dot-org |
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=head1 APPENDIX |
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67
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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72
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# Let the code begin... |
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74
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package Bio::TreeIO::newick; |
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use strict; |
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76
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77
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54
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use Bio::Event::EventGeneratorI; |
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348
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79
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use base qw(Bio::TreeIO Bio::TreeIO::NewickParser); |
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5825
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80
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81
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=head2 new |
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83
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Title : new |
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Args : -print_count => boolean default is false |
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-bootstrap_style => set the bootstrap style (one of nobranchlength, |
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molphy, traditional) |
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-order_by => set the order by sort method |
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89
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See L |
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=cut |
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93
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sub _initialize { |
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680
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680
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548
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my $self = shift; |
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95
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680
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1450
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$self->SUPER::_initialize(@_); |
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680
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1653
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my ( $print_count ) = $self->_rearrange( |
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[ |
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qw(PRINT_COUNT) |
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], |
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@_ |
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); |
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680
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2479
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$self->print_tree_count( $print_count || 0 ); |
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680
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852
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return; |
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} |
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105
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106
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=head2 next_tree |
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107
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108
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Title : next_tree |
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Usage : my $tree = $treeio->next_tree |
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Function: Gets the next tree in the stream |
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Returns : L |
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Args : none |
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114
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=cut |
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116
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sub next_tree { |
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155
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155
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1
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my ($self) = @_; |
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155
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537
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local $/ = ";\n"; |
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155
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100
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return unless $_ = $self->_readline; |
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121
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153
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s/[\r\n]//gs; |
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153
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my $score; |
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153
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0
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my $despace = sub { my $dirty = shift; $dirty =~ s/\s+//gs; return $dirty }; |
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124
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my $dequote = sub { |
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0
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my $dirty = shift; |
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0
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$dirty =~ s/^"?\s*(.+?)\s*"?$/$1/; |
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0
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return $dirty; |
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153
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}; |
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153
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s/([^"]*)(".+?")([^"]*)/$despace->($1) . $dequote->($2) . $despace->($3)/egsx; |
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0
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0
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130
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131
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153
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100
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398
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if (s/^\s*\[([^\]]+)\]//) { |
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2
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my $match = $1; |
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2
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$match =~ s/\s//g; |
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2
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$match =~ s/lh\=//; |
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2
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if ( $match =~ /([-\d\.+]+)/ ) { |
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2
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$score = $1; |
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} |
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138
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} |
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140
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153
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355
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$self->_eventHandler->start_document; |
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142
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# Call the parse_newick method as defined in NewickParser.pm |
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153
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$self->parse_newick($_); |
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145
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153
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my $tree = $self->_eventHandler->end_document; |
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147
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# Add the tree score afterwards if it exists. |
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153
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if (defined $tree) { |
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153
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$tree->score($score); |
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153
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return $tree; |
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} |
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} |
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153
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154
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# Returns the default set of parsing & writing parameters for the Newick format. |
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sub get_default_params { |
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680
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680
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0
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my $self = shift; |
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158
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return { |
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680
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newline_each_node => 0, |
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order_by => '', # ??? |
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bootstrap_style => 'traditional', # Can be 'traditional', 'molphy', 'nobranchlength' |
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162
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internal_node_id => 'id', # Can be 'id' or 'bootstrap' |
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163
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164
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no_branch_lengths => 0, |
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165
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no_bootstrap_values => 0, |
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166
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no_internal_node_labels => 0 |
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167
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}; |
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168
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} |
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169
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170
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171
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=head2 write_tree |
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172
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173
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Title : write_tree |
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174
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Usage : $treeio->write_tree($tree); |
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175
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Function: Write a tree out to data stream in newick/phylip format |
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176
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Returns : none |
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177
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Args : L object |
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178
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179
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=cut |
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180
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181
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sub write_tree { |
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182
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541
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541
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1
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570
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my ( $self, @trees ) = @_; |
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183
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541
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50
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688
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if ( $self->print_tree_count ) { |
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184
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0
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0
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$self->_print( sprintf( " %d\n", scalar @trees ) ); |
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185
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} |
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186
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187
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541
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889
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my $params = $self->get_params; |
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188
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189
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541
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652
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foreach my $tree (@trees) { |
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190
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541
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50
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33
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3709
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if ( !defined $tree |
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33
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191
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|| ref($tree) =~ /ARRAY/i |
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192
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|| !$tree->isa('Bio::Tree::TreeI') ) |
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193
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{ |
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194
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0
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0
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$self->throw( |
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195
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"Calling write_tree with non Bio::Tree::TreeI object\n"); |
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196
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} |
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197
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541
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1085
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my @data = $self->_write_tree_Helper( $tree->get_root_node, $params); |
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198
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541
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2201
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$self->_print( join( ',', @data ).";" ); |
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199
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} |
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200
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201
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541
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50
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33
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844
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$self->flush if $self->_flush_on_write && defined $self->_fh; |
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202
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541
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846
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return; |
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203
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} |
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204
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205
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sub _write_tree_Helper { |
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206
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3290
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3290
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2302
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my $self = shift; |
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207
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3290
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2433
|
my ( $node, $params ) = @_; |
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208
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3290
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1921
|
my @data; |
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209
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210
|
3290
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4737
|
foreach my $n ( $node->each_Descendent($params->{order_by}) ) { |
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211
|
2749
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|
3487
|
push @data, $self->_write_tree_Helper( $n, $params ); |
|
212
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} |
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213
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214
|
3290
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4472
|
my $label = $self->_node_as_string($node,$params); |
|
215
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216
|
3290
|
100
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|
3949
|
if ( scalar(@data) >= 1) { |
|
217
|
1355
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|
1549
|
$data[0] = "(" . $data[0]; |
|
218
|
1355
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|
1068
|
$data[-1] .= ")"; |
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219
|
1355
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|
1359
|
$data[-1] .= $label; |
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220
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|
|
} else { |
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221
|
1935
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|
1709
|
push @data, $label; |
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222
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} |
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223
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224
|
3290
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|
5720
|
return @data; |
|
225
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} |
|
226
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227
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|
|
sub _node_as_string { |
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228
|
3290
|
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|
3290
|
|
2230
|
my $self = shift; |
|
229
|
3290
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|
1951
|
my $node = shift; |
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230
|
3290
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|
2029
|
my $params = shift; |
|
231
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232
|
3290
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|
2368
|
my $label_stringbuffer = ''; |
|
233
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|
234
|
3290
|
100
|
66
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|
|
6764
|
if ($params->{no_bootstrap_values} != 1 && |
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|
|
50
|
100
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66
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100
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|
235
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|
|
!$node->is_Leaf && |
|
236
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|
|
defined $node->bootstrap && |
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237
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|
|
$params->{bootstrap_style} eq 'traditional' && |
|
238
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|
|
$params->{internal_node_id} eq 'bootstrap') { |
|
239
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|
|
# If we're an internal node and we're using 'traditional' bootstrap style, |
|
240
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|
|
# we output the bootstrap instead of any label. |
|
241
|
5
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|
7
|
my $bootstrap = $node->bootstrap; |
|
242
|
5
|
50
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|
|
7
|
$label_stringbuffer .= $bootstrap if (defined $bootstrap); |
|
243
|
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|
|
} elsif ($params->{no_internal_node_labels} != 1) { |
|
244
|
3285
|
|
|
|
|
4050
|
my $id = $node->id; |
|
245
|
3285
|
100
|
|
|
|
5020
|
$label_stringbuffer .= $id if( defined $id ); |
|
246
|
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|
|
} |
|
247
|
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|
248
|
3290
|
50
|
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|
4539
|
if ($params->{no_branch_lengths} != 1) { |
|
249
|
3290
|
|
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|
|
4002
|
my $blen = $node->branch_length; |
|
250
|
3290
|
100
|
|
|
|
9288
|
$label_stringbuffer .= ":". $blen if (defined $blen); |
|
251
|
|
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|
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|
|
} |
|
252
|
|
|
|
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|
|
253
|
3290
|
50
|
|
|
|
4249
|
if ($params->{bootstrap_style} eq 'molphy') { |
|
254
|
0
|
|
|
|
|
0
|
my $bootstrap = $node->bootstrap; |
|
255
|
0
|
0
|
|
|
|
0
|
$label_stringbuffer .= "[$bootstrap]" if (defined $bootstrap); |
|
256
|
|
|
|
|
|
|
} |
|
257
|
|
|
|
|
|
|
|
|
258
|
3290
|
50
|
|
|
|
3778
|
if ($params->{newline_each_node} == 1) { |
|
259
|
0
|
|
|
|
|
0
|
$label_stringbuffer .= "\n"; |
|
260
|
|
|
|
|
|
|
} |
|
261
|
|
|
|
|
|
|
|
|
262
|
3290
|
|
|
|
|
3516
|
return $label_stringbuffer; |
|
263
|
|
|
|
|
|
|
} |
|
264
|
|
|
|
|
|
|
|
|
265
|
|
|
|
|
|
|
|
|
266
|
|
|
|
|
|
|
=head2 print_tree_count |
|
267
|
|
|
|
|
|
|
|
|
268
|
|
|
|
|
|
|
Title : print_tree_count |
|
269
|
|
|
|
|
|
|
Usage : $obj->print_tree_count($newval) |
|
270
|
|
|
|
|
|
|
Function: Get/Set flag for printing out the tree count (paml,protml way) |
|
271
|
|
|
|
|
|
|
Returns : value of print_tree_count (a scalar) |
|
272
|
|
|
|
|
|
|
Args : on set, new value (a scalar or undef, optional) |
|
273
|
|
|
|
|
|
|
|
|
274
|
|
|
|
|
|
|
=cut |
|
275
|
|
|
|
|
|
|
|
|
276
|
|
|
|
|
|
|
sub print_tree_count { |
|
277
|
1221
|
|
|
1221
|
1
|
905
|
my $self = shift; |
|
278
|
1221
|
100
|
|
|
|
2317
|
return $self->{'_print_tree_count'} = shift if @_; |
|
279
|
541
|
|
50
|
|
|
1823
|
return $self->{'_print_tree_count'} || 0; |
|
280
|
|
|
|
|
|
|
} |
|
281
|
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
=head2 bootstrap_style |
|
283
|
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
Title : bootstrap_style |
|
285
|
|
|
|
|
|
|
Usage : $obj->bootstrap_style($newval) |
|
286
|
|
|
|
|
|
|
Function: A description of how bootstraps and branch lengths are |
|
287
|
|
|
|
|
|
|
written, as the ID part of the internal node or else in [] |
|
288
|
|
|
|
|
|
|
in the branch length (Molphy-like; I am sure there is a |
|
289
|
|
|
|
|
|
|
better name for this but am not sure where to go for some |
|
290
|
|
|
|
|
|
|
sort of format documentation) |
|
291
|
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
If no branch lengths are requested then no bootstraps are usually |
|
293
|
|
|
|
|
|
|
written (unless someone REALLY wants this functionality...) |
|
294
|
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
Can take on strings which contain the possible values of |
|
296
|
|
|
|
|
|
|
'nobranchlength' --> don't draw any branch lengths - this |
|
297
|
|
|
|
|
|
|
is helpful if you don't want to have to |
|
298
|
|
|
|
|
|
|
go through and delete branch len on all nodes |
|
299
|
|
|
|
|
|
|
'molphy' --> draw bootstraps (100) like |
|
300
|
|
|
|
|
|
|
(A:0.11,B:0.22):0.33[100]; |
|
301
|
|
|
|
|
|
|
'traditional' --> draw bootstraps (100) like |
|
302
|
|
|
|
|
|
|
(A:0.11,B:0.22)100:0.33; |
|
303
|
|
|
|
|
|
|
Returns : value of bootstrap_style (a scalar) |
|
304
|
|
|
|
|
|
|
Args : on set, new value (a scalar or undef, optional) |
|
305
|
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
=cut |
|
307
|
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
sub bootstrap_style { |
|
309
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
310
|
0
|
|
|
|
|
|
my $val = shift; |
|
311
|
0
|
0
|
|
|
|
|
if ( defined $val ) { |
|
312
|
|
|
|
|
|
|
|
|
313
|
0
|
0
|
|
|
|
|
if ( $val !~ /^nobranchlength|molphy|traditional/i ) { |
|
314
|
0
|
|
|
|
|
|
$self->warn( |
|
315
|
|
|
|
|
|
|
"requested an unknown bootstrap style $val, expect one of nobranchlength,molphy,traditional, not updating value.\n" |
|
316
|
|
|
|
|
|
|
); |
|
317
|
|
|
|
|
|
|
} |
|
318
|
|
|
|
|
|
|
else { |
|
319
|
0
|
|
|
|
|
|
$self->{'_bootstrap_style'} = $val; |
|
320
|
|
|
|
|
|
|
} |
|
321
|
|
|
|
|
|
|
} |
|
322
|
0
|
|
0
|
|
|
|
return $self->{'_bootstrap_style'} || 'traditional'; |
|
323
|
|
|
|
|
|
|
} |
|
324
|
|
|
|
|
|
|
|
|
325
|
|
|
|
|
|
|
=head2 order_by |
|
326
|
|
|
|
|
|
|
|
|
327
|
|
|
|
|
|
|
Title : order_by |
|
328
|
|
|
|
|
|
|
Usage : $obj->order_by($newval) |
|
329
|
|
|
|
|
|
|
Function: Allow node order to be specified (typically "alpha") |
|
330
|
|
|
|
|
|
|
See L |
|
331
|
|
|
|
|
|
|
Returns : value of order_by (a scalar) |
|
332
|
|
|
|
|
|
|
Args : on set, new value (a scalar or undef, optional) |
|
333
|
|
|
|
|
|
|
|
|
334
|
|
|
|
|
|
|
=cut |
|
335
|
|
|
|
|
|
|
|
|
336
|
|
|
|
|
|
|
sub order_by { |
|
337
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
338
|
|
|
|
|
|
|
|
|
339
|
0
|
0
|
|
|
|
|
return $self->{'order_by'} = shift if @_; |
|
340
|
0
|
|
|
|
|
|
return $self->{'order_by'}; |
|
341
|
|
|
|
|
|
|
} |
|
342
|
|
|
|
|
|
|
|
|
343
|
|
|
|
|
|
|
1; |