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package App::SimulateReads::Command::QualityDB::Add; |
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# ABSTRACT: qualitydb subcommand class. Add a quality profile to the database. |
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use App::SimulateReads::Base 'class'; |
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extends 'App::SimulateReads::Command::QualityDB'; |
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our $VERSION = '0.06'; # VERSION |
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use constant { |
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TYPE_OPT => ['raw', 'fastq'] |
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}; |
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override 'opt_spec' => sub { |
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super, |
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'verbose|v', |
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'quality-profile|q=s', |
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'read-size|r=i', |
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'source|s=s' |
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}; |
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sub _default_opt { |
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'verbose' => 0, |
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'type' => 'fastq', |
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'source' => 'not defined' |
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} |
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sub validate_args { |
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my ($self, $args) = @_; |
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my $file = shift @$args; |
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# Mandatory file |
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if (not defined $file) { |
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die "Missing file (a quality file or fastq file)\n"; |
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} |
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# Is it really a file? |
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if (not -f $file) { |
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die "<$file> is not a file. Please, give me a valid quality or fastq file\n"; |
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} |
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die "Too many arguments: '@$args'\n" if @$args; |
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} |
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sub validate_opts { |
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my ($self, $opts) = @_; |
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my %default_opt = $self->_default_opt; |
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$self->fill_opts($opts, \%default_opt); |
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if (not exists $opts->{'quality-profile'}) { |
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die "Option 'quality-profile' not defined\n"; |
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} |
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if (not exists $opts->{'read-size'}) { |
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die "Option 'read-size' not defined\n"; |
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} |
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if ($opts->{'read-size'} <= 0) { |
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die "Option 'read-size' requires an integer greater than zero\n"; |
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} |
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} |
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sub execute { |
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my ($self, $opts, $args) = @_; |
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my $file = shift @$args; |
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my %default_opt = $self->_default_opt; |
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$self->fill_opts($opts, \%default_opt); |
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# Set if user wants a verbose log |
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$LOG_VERBOSE = $opts->{verbose}; |
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# Set the type of file |
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if ($file !~ /.+\.(fastq)(\.gz)?$/) { |
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$opts->{type} = 'raw'; |
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} |
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# Go go go |
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log_msg "Inserting $opts->{'quality-profile'}:$opts->{'read-size'} from $file ..."; |
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$self->insertdb( |
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$file, |
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$opts->{'quality-profile'}, |
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$opts->{'read-size'}, |
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$opts->{'source'}, |
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1, |
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$opts->{'type'} |
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); |
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log_msg "Done!"; |
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} |
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__END__ |
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=pod |
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=encoding UTF-8 |
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98
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=head1 NAME |
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100
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App::SimulateReads::Command::QualityDB::Add - qualitydb subcommand class. Add a quality profile to the database. |
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101
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102
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=head1 VERSION |
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104
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version 0.06 |
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106
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=head1 SYNOPSIS |
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108
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simulate_reads qualitydb add -q <entry name> -r <size> [-s <source>] FILE |
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Arguments: |
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a quality-profile file (fastq or a matrix with only quality entries) |
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Options: |
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-h, --help brief help message |
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-M, --man full documentation |
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-v, --verbose print log messages |
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-q, --quality-profile quality-profile name for the database [required] |
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-r, --read-size the read-size to be used for the quality [required, Integer] |
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-s, --source qaulity-profile source detail for database |
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=head1 DESCRIPTION |
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B<simulate_reads> will read the given input file and do something |
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useful with the contents thereof. |
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=head1 AUTHOR |
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Thiago L. A. Miller <tmiller@mochsl.org.br> |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2017 by Teaching and Research Institute from SÃrio-Libanês Hospital. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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=cut |