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package App::SimulateReads::Fastq; |
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# ABSTRACT: Base class to simulate fastq entries |
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use App::SimulateReads::Base 'class'; |
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use App::SimulateReads::Quality; |
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our $VERSION = '0.05'; # VERSION |
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#------------------------------------------------------------------------------- |
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# Moose attributes |
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#------------------------------------------------------------------------------- |
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has 'quality_profile' => (is => 'ro', isa => 'My:QualityP', required => 1, coerce => 1); |
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has 'read_size' => (is => 'ro', isa => 'My:IntGt0', required => 1); |
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has '_quality' => ( |
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is => 'ro', |
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isa => 'App::SimulateReads::Quality', |
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builder => '_build_quality', |
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lazy_build => 1, |
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handles => [qw{ gen_quality }] |
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); |
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sub BUILD { |
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my $self = shift; |
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## Just to ensure that the lazy attributes are built before &new returns |
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$self->_quality; |
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} |
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#=== CLASS METHOD ============================================================ |
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# CLASS: Fast |
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# METHOD: _build_quality (BUILDER) |
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# PARAMETERS: Void |
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# RETURNS: Quality obj |
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# DESCRIPTION: Build Quality object |
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# THROWS: no exceptions |
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# COMMENTS: none |
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# SEE ALSO: n/a |
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#=============================================================================== |
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sub _build_quality { |
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my $self = shift; |
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App::SimulateReads::Quality->new( |
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quality_profile => $self->quality_profile, |
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read_size => $self->read_size |
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); |
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} ## --- end sub _build_quality |
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#=== CLASS METHOD ============================================================ |
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# CLASS: Fast |
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# METHOD: sprintf_fastq |
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# PARAMETERS: $header_ref Ref Str, $seq_ref Ref Str |
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# RETURNS: $fastq Ref Str |
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# DESCRIPTION: Fastq entry template |
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# THROWS: no exceptions |
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# COMMENTS: none |
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# SEE ALSO: n/a |
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#=============================================================================== |
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sub fastq_template { |
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my ($self, $header_ref, $seq_ref) = @_; |
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my $quality_ref = $self->gen_quality; |
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my $fastq = "\@$$header_ref\n"; |
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$fastq .= "$$seq_ref\n"; |
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$fastq .= "+\n"; |
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$fastq .= "$$quality_ref"; |
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return \$fastq; |
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} ## --- end sub fastq_template |
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__END__ |
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=pod |
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=encoding UTF-8 |
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74
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=head1 NAME |
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76
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App::SimulateReads::Fastq - Base class to simulate fastq entries |
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78
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=head1 VERSION |
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version 0.05 |
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=head1 AUTHOR |
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84
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Thiago L. A. Miller <tmiller@mochsl.org.br> |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2017 by Teaching and Research Institute from SÃrio-Libanês Hospital. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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94
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=cut |