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133198
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use strict; |
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1
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2
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1
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25
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2
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1
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1
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5
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use warnings; |
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1
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2
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1
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31
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3
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4
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package App::RecordStream::Operation::fromgff3; |
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1
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1
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5
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use base qw(App::RecordStream::Operation); |
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1
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2
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1
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58
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6
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7
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1
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1
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383
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use Bio::GFF3::LowLevel qw< gff3_parse_feature >; |
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1
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2537
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1
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257
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8
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9
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sub init { |
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10
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1
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1
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0
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481
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my $this = shift; |
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11
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1
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2
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my $args = shift; |
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12
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1
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2
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my $options = {}; |
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13
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1
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7
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$this->parse_options($args, $options); # Ensures we pick up the automatic options |
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14
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} |
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15
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16
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sub accept_line { |
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17
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20
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20
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0
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1139
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my $this = shift; |
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18
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20
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35
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my $line = shift; |
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19
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20
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# Skip headers, comments, and empty lines |
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21
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20
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100
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227
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return 1 if $line =~ /^#|^$/; |
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22
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23
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11
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30
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my $feature = gff3_parse_feature($line); |
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24
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25
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11
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1257
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$this->push_record( App::RecordStream::Record->new($feature) ); |
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26
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11
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245
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return 1; |
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27
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} |
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28
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29
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sub usage { |
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30
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0
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0
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0
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my $this = shift; |
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31
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0
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my $options = []; |
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32
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0
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my $args_string = $this->options_string($options); |
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33
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34
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0
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return <
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35
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Usage: recs fromgff3 [] |
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36
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__FORMAT_TEXT__ |
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37
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Each line of input (or lines of ) is parsed as a GFF3 (General |
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38
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Feature Format version 3) formatted record to produce a recs output record. |
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39
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40
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The output records will contain the following fields: |
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41
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__FORMAT_TEXT__ |
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42
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43
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seq_id source type start end score strand phase attributes |
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44
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45
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__FORMAT_TEXT__ |
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46
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Additional feature attributes are parsed into the "attributes" key, which is a |
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47
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hash keyed by the attribute name. The values are arrays. |
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48
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__FORMAT_TEXT__ |
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49
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50
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Refer to the GFF3 spec for field details: |
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51
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https://github.com/The-Sequence-Ontology/Specifications/blob/master/gff3.md |
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52
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53
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Arguments: |
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54
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$args_string |
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55
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56
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Examples: |
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57
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Parse GFF3 and filter to just mRNA features: |
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58
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recs fromgff3 hg38.gff | recs grep '{{type}} eq "mRNA"' |
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59
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60
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USAGE |
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61
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} |
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62
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63
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1; |